Though it may be old fashioned, we here at our hospital can still only do AFB staining for detecting AFB in sputum samples. I am not a lab person, but as I understand it, the gold standard protocol involves digestion, decontamination, and centrifugation (using a 50 mL size centrifuge). The lab is not even processing the specimens but only taking sputum and directly smearing it on the slide and doing the staining. As expected, the yield for positive bacilli is quite low (actually zero). The lab does have a 10 mL size centrifuge (and will not be able to purchase a 50 mL one), my question to everyone is: does anyone have a protocol using a smaller size centrifuge?
Thanks for any help!